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Experiment Id: E234
Experiment Name: MyoD-/-, Time Course
Description: Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time course: 0 hr, 6 hr, 12 hr, 18 hr, 24 hr, 36 hr, 48 hr, 3 days, 4 days, 7 days.
Note that the Triplicate1 sample = MyoD-/-1999; Triplicate 2 sample = MyoD-/-p6; Triplicate 3 sample = MyoD-/-p10.
Owner: Dr. Michael Rudnicki
Network Release Date: 2006-09-08 00:00:00
Public Release Date: 2006-09-08 00:00:00
Workflow State: RELEASED
External link: GEO Series Accession GSE10430
Download Expression Values For Experiment
| Property |
Value |
| SCGP ID |
S343 |
| Name |
MyoD -/-, 0hr |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 0 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-, 1999, 0hr |
3325.8 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1739A.zip |
MG_U74Av2 |
GSM263821 |
 |
| AFFY#1739B.zip |
MG_U74Bv2 |
GSM263822 |
|
| AFFY#1739C.zip |
MG_U74Cv2 |
GSM263823 |
|
|
|
| MyoD-/-, P6, 0hr |
4327.4 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1679A.zip |
MG_U74Av2 |
GSM263824 |
|
| AFFY#1679B.zip |
MG_U74Bv2 |
GSM263825 |
|
| AFFY#1679C.zip |
MG_U74Cv2 |
GSM263826 |
|
|
|
| MyoD-/-, P10, 0hr |
1468.0 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1653A.zip |
MG_U74Av2 |
GSM263827 |
|
| AFFY#1653B.zip |
MG_U74Bv2 |
GSM263828 |
|
| AFFY#1653C.zip |
MG_U74Cv2 |
GSM263829 |
|
|
|
| Property |
Value |
| SCGP ID |
S344 |
| Name |
MyoD -/-, 6hr |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 6 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-, 1999, 6hr |
2830.4 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1740A.zip |
MG_U74Av2 |
GSM263830 |
|
| AFFY#1740B.zip |
MG_U74Bv2 |
GSM263831 |
|
| AFFY#1740C.zip |
MG_U74Cv2 |
GSM263832 |
|
|
|
| MyoD-/-, P6, 6hr |
2205.8 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1680A.zip |
MG_U74Av2 |
GSM263833 |
|
| AFFY#1680B.zip |
MG_U74Bv2 |
GSM263834 |
|
| AFFY#1680C.zip |
MG_U74Cv2 |
GSM263835 |
|
|
|
| MyoD-/-, P10, 6hr |
2606.6 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1654A.zip |
MG_U74Av2 |
GSM263836 |
|
| AFFY#1654B.zip |
MG_U74Bv2 |
GSM263837 |
|
| AFFY#1654C.zip |
MG_U74Cv2 |
GSM263838 |
|
|
|
| Property |
Value |
| SCGP ID |
S345 |
| Name |
MyoD -/-, 12hr |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 12 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-, 1999, 12hr |
2830.4 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1741A.zip |
MG_U74Av2 |
GSM263839 |
|
| AFFY#1741B.zip |
MG_U74Bv2 |
GSM263840 |
|
| AFFY#1741C.zip |
MG_U74Cv2 |
GSM263841 |
|
|
|
| MyoD-/-, P6, 12hr |
2205.8 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1681A.zip |
MG_U74Av2 |
GSM263842 |
|
| AFFY#1681B.zip |
MG_U74Bv2 |
GSM263843 |
|
| AFFY#1681C.zip |
MG_U74Cv2 |
GSM263844 |
|
|
|
| MyoD-/-, P10, 12hr |
2606.6 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1655A.zip |
MG_U74Av2 |
GSM263845 |
|
| AFFY#1655B.zip |
MG_U74Bv2 |
GSM263846 |
|
| AFFY#1655C.zip |
MG_U74Cv2 |
GSM263847 |
|
|
|
| Property |
Value |
| SCGP ID |
S346 |
| Name |
MyoD -/-, 18hr |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 18 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-, 1999, 18hr |
1419.4 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1742A.zip |
MG_U74Av2 |
GSM263848 |
|
| AFFY#1742B.zip |
MG_U74Bv2 |
GSM263849 |
|
| AFFY#1742C.zip |
MG_U74Cv2 |
GSM263850 |
|
|
|
| MyoD-/-, P6, 18hr |
1960.2 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1682A.zip |
MG_U74Av2 |
GSM263851 |
|
| AFFY#1682B.zip |
MG_U74Bv2 |
GSM263852 |
|
| AFFY#1682C.zip |
MG_U74Cv2 |
GSM263853 |
|
|
|
| MyoD-/-, P10, 18hr |
2226.4 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1656A.zip |
MG_U74Av2 |
GSM263854 |
|
| AFFY#1656B.zip |
MG_U74Bv2 |
GSM263855 |
|
| AFFY#1656C.zip |
MG_U74Cv2 |
GSM263856 |
|
|
|
| Property |
Value |
| SCGP ID |
S347 |
| Name |
MyoD -/-, 24hr |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 24 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-, 1999, 24hr |
2583.8 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1743A.zip |
MG_U74Av2 |
GSM263857 |
|
| AFFY#1743B.zip |
MG_U74Bv2 |
GSM263858 |
|
| AFFY#1743C.zip |
MG_U74Cv2 |
GSM263859 |
|
|
|
| MyoD-/-, P6, 24hr |
1879.2 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1683A.zip |
MG_U74Av2 |
GSM263860 |
|
| AFFY#1683B.zip |
MG_U74Bv2 |
GSM263861 |
|
| AFFY#1683C.zip |
MG_U74Cv2 |
GSM263862 |
|
|
|
| MyoD-/-, P10, 24hr |
2229.8 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1657A.zip |
MG_U74Av2 |
GSM263863 |
|
| AFFY#1657B.zip |
MG_U74Bv2 |
GSM263864 |
|
| AFFY#1657C.zip |
MG_U74Cv2 |
GSM263865 |
|
|
|
| Property |
Value |
| SCGP ID |
S348 |
| Name |
MyoD -/-, 36hr |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 36 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-, 1999, 36hr |
1709.4 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1669A.zip |
MG_U74Av2 |
GSM263866 |
|
| AFFY#1669B.zip |
MG_U74Bv2 |
GSM263867 |
|
| AFFY#1669C.zip |
MG_U74Cv2 |
GSM263868 |
|
|
|
| MyoD-/-, P6, 36hr |
1298.0 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1684A.zip |
MG_U74Av2 |
GSM263869 |
|
| AFFY#1684B.zip |
MG_U74Bv2 |
GSM263870 |
|
| AFFY#1684C.zip |
MG_U74Cv2 |
GSM263871 |
|
|
|
| MyoD-/-, P10, 36hr |
1586.8 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1658A.zip |
MG_U74Av2 |
GSM263872 |
|
| AFFY#1658B.zip |
MG_U74Bv2 |
GSM263873 |
|
| AFFY#1658C.zip |
MG_U74Cv2 |
GSM263874 |
|
|
|
| Property |
Value |
| SCGP ID |
S349 |
| Name |
MyoD -/-, 48hr |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 48 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-, 1999, 48hr |
1110.6 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1670A.zip |
MG_U74Av2 |
GSM263875 |
|
| AFFY#1670B.zip |
MG_U74Bv2 |
GSM263876 |
|
| AFFY#1670C.zip |
MG_U74Cv2 |
GSM263877 |
|
|
|
| MyoD-/-, P6, 48hr |
1086.4 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1685A.zip |
MG_U74Av2 |
GSM263878 |
|
| AFFY#1685B.zip |
MG_U74Bv2 |
GSM263879 |
|
| AFFY#1685C.zip |
MG_U74Cv2 |
GSM263880 |
|
|
|
| MyoD-/-, P10, 48hr |
1294.3 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1659A.zip |
MG_U74Av2 |
GSM263881 |
|
| AFFY#1659B.zip |
MG_U74Bv2 |
GSM263882 |
|
| AFFY#1659C.zip |
MG_U74Cv2 |
GSM263883 |
|
|
|
| Property |
Value |
| SCGP ID |
S350 |
| Name |
MyoD -/-, 3 days |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 3 day |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-,1999, 3d |
1937.6 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1744A.zip |
MG_U74Av2 |
GSM263884 |
|
| AFFY#1744B.zip |
MG_U74Bv2 |
GSM263885 |
|
| AFFY#1744C.zip |
MG_U74Cv2 |
GSM263886 |
|
|
|
| MyoD-/-, P10, 3d |
1336.7 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1660A.zip |
MG_U74Av2 |
GSM263887 |
|
| AFFY#1660B.zip |
MG_U74Bv2 |
GSM263888 |
|
| AFFY#1660C.zip |
MG_U74Cv2 |
GSM263889 |
|
|
|
| MyoD-/-,P6, 3d |
1652.8 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1686A.zip |
MG_U74Av2 |
GSM263890 |
|
| AFFY#1686B.zip |
MG_U74Bv2 |
GSM263891 |
|
| AFFY#1686C.zip |
MG_U74Cv2 |
GSM263892 |
|
|
|
| Property |
Value |
| SCGP ID |
S351 |
| Name |
MyoD -/-, 4 days |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 4 day |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-, 1999, 4d |
1815.8 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1745A.zip |
MG_U74Av2 |
GSM263893 |
|
| AFFY#1745B.zip |
MG_U74Bv2 |
GSM263894 |
|
| AFFY#1745C.zip |
MG_U74Cv2 |
GSM263895 |
|
|
|
| MyoD-/-, P6, 4d |
2485.4 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1687A.zip |
MG_U74Av2 |
GSM263896 |
|
| AFFY#1687B.zip |
MG_U74Bv2 |
GSM263897 |
|
| AFFY#1687C.zip |
MG_U74Cv2 |
GSM263898 |
|
|
|
| MyoD-/-, P10, 4d |
1130.3 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1661A.zip |
MG_U74Av2 |
GSM263899 |
|
| AFFY#1661B.zip |
MG_U74Bv2 |
GSM263900 |
|
| AFFY#1661C.zip |
MG_U74Cv2 |
GSM263901 |
|
|
|
| Property |
Value |
| SCGP ID |
S352 |
| Name |
MyoD -/-, 7 days |
| Description |
Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 7 day |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD-/- |
| Gender |
Male |
| Karyotype |
normal |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| Cell Purification Method |
cultured |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD-/-,1999, 7d |
1374.0 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1775A.zip |
MG_U74Av2 |
GSM263902 |
|
| AFFY#1775B.zip |
MG_U74Bv2 |
GSM263903 |
|
| AFFY#1775C.zip |
MG_U74Cv2 |
GSM263904 |
|
|
|
| MyoD-/-,P6, 7d |
2347.2 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| AFFY#1688A.zip |
MG_U74Av2 |
GSM263905 |
|
| AFFY#1688B.zip |
MG_U74Bv2 |
GSM263906 |
|
| AFFY#1688C.zip |
MG_U74Cv2 |
GSM263907 |
|
|
|
| MyoD-/-,P10, 7d |
1545.2 ng/µL |
27µL |
More than 2 x 10E+6 |
| Name |
Type |
GEO | Action |
| Affy#1738A.zip |
MG_U74Av2 |
GSM263908 |
|
| Affy#1738B.zip |
MG_U74Bv2 |
GSM263909 |
|
| Affy#1738C.zip |
MG_U74Cv2 |
GSM263910 |
|
|
|
|
