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Experiment Id: E233
Experiment Name: MyoD wt, Time Course
Description: Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time course: 0 hr, 6 hr, 12 hr, 18 hr, 24 hr, 36 hr, 48 hr, 3 days, 4 days, 7 days.
Owner: Dr. Michael Rudnicki
Network Release Date: 2006-08-27 00:00:00
Public Release Date: 2006-08-27 00:00:00
Workflow State: RELEASED
External link: GEO Series Accession GSE10424
Download Expression Values For Experiment
| Property |
Value |
| SCGP ID |
S333 |
| Name |
MyoD wt, Time Course 0hr |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time course: 0 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 0hr |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1378A.zip |
MG_U74Av2 |
GSM263671 |
 |
| AFFY#1378B.zip |
MG_U74Bv2 |
GSM263672 |
|
| AFFY#1378C.zip |
MG_U74Cv2 |
GSM263673 |
|
|
|
| MyoD, wtp16, 0hr |
1170.2 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1776_1A.zip |
MG_U74Av2 |
GSM263674 |
|
| AFFY#1776_1B.zip |
MG_U74Bv2 |
GSM263675 |
|
| AFFY#1776_1C.zip |
MG_U74Cv2 |
GSM263676 |
|
|
|
| MyoD, wt841, 0hr |
2297.8 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1796A.zip |
MG_U74Av2 |
GSM263677 |
|
| AFFY#1796B.zip |
MG_U74Bv2 |
GSM263678 |
|
| AFFY#1796C.zip |
MG_U74Cv2 |
GSM263679 |
|
|
|
| Property |
Value |
| SCGP ID |
S334 |
| Name |
MyoD wt, Time Course 6hr |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time course: 6 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 6hr |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1379A.zip |
MG_U74Av2 |
GSM263680 |
|
| AFFY#1379B.zip |
MG_U74Bv2 |
GSM263681 |
|
| AFFY#1379C.zip |
MG_U74Cv2 |
GSM263682 |
|
|
|
| MyoD, wtp16, 6hr |
1393.0 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1777_1A.zip |
MG_U74Av2 |
GSM263683 |
|
| AFFY#1777_1B.zip |
MG_U74Bv2 |
GSM263684 |
|
| AFFY#1777_1C.zip |
MG_U74Cv2 |
GSM263685 |
|
|
|
| MyoD, wt841, 6hr |
1212.4 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1797A.zip |
MG_U74Av2 |
GSM263686 |
|
| AFFY#1797B.zip |
MG_U74Bv2 |
GSM263687 |
|
| AFFY#1797C.zip |
MG_U74Cv2 |
GSM263688 |
|
|
|
| Property |
Value |
| SCGP ID |
S335 |
| Name |
MyoD wt, Time Course 12hr |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time course: 12 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 12hr |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1380A.zip |
MG_U74Av2 |
GSM263689 |
|
| AFFY#1380B.zip |
MG_U74Bv2 |
GSM263690 |
|
| AFFY#1380C.zip |
MG_U74Cv2 |
GSM263691 |
|
|
|
| MyoD, wtp16, 12hr |
1760.6 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1778_1A.zip |
MG_U74Av2 |
GSM263692 |
|
| AFFY#1778_1B.zip |
MG_U74Bv2 |
GSM263693 |
|
| AFFY#1778_1C.zip |
MG_U74Cv2 |
GSM263694 |
|
|
|
| MyoD, wt841, 12hr |
2012.8 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1798A.zip |
MG_U74Av2 |
GSM263695 |
|
| AFFY#1798B.zip |
MG_U74Bv2 |
GSM263696 |
|
| AFFY#1798C.zip |
MG_U74Cv2 |
GSM263697 |
|
|
|
| Property |
Value |
| SCGP ID |
S336 |
| Name |
MyoD wt, Time Course 18hr |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 18 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 18hr |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1381A.zip |
MG_U74Av2 |
GSM263698 |
|
| AFFY#1381B.zip |
MG_U74Bv2 |
GSM263699 |
|
| AFFY#1381C.zip |
MG_U74Cv2 |
GSM263700 |
|
|
|
| MyoD, wtp16, 18hr |
1593.0 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1779_1A.zip |
MG_U74Av2 |
GSM263701 |
|
| AFFY#1779_1B.zip |
MG_U74Bv2 |
GSM263702 |
|
| AFFY#1779_1C.zip |
MG_U74Cv2 |
GSM263703 |
|
|
|
| MyoD, wt841, 18hr |
1597.2 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1799A.zip |
MG_U74Av2 |
GSM263704 |
|
| AFFY#1799B.zip |
MG_U74Bv2 |
GSM263705 |
|
| AFFY#1799C.zip |
MG_U74Cv2 |
GSM263706 |
|
|
|
| Property |
Value |
| SCGP ID |
S337 |
| Name |
MyoD wt, Time Course 24hr |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 24 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 24hr |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1382A.zip |
MG_U74Av2 |
GSM263707 |
|
| AFFY#1382B.zip |
MG_U74Bv2 |
GSM263708 |
|
| AFFY#1382C.zip |
MG_U74Cv2 |
GSM263709 |
|
|
|
| MyoD, wtp16, 24hr |
1624.4 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1780_1A.zip |
MG_U74Av2 |
GSM263710 |
|
| AFFY#1780_1B.zip |
MG_U74Bv2 |
GSM263711 |
|
| AFFY#1780_1C.zip |
MG_U74Cv2 |
GSM263712 |
|
|
|
| MyoD, wt841, 24hr |
1830.8 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1800A.zip |
MG_U74Av2 |
GSM263713 |
|
| AFFY#1800B.zip |
MG_U74Bv2 |
GSM263714 |
|
| AFFY#1800C.zip |
MG_U74Cv2 |
GSM263715 |
|
|
|
| Property |
Value |
| SCGP ID |
S338 |
| Name |
MyoD wt, Time Course 36hr |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 36 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 36hr |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1383A.zip |
MG_U74Av2 |
GSM263716 |
|
| AFFY#1383B.zip |
MG_U74Bv2 |
GSM263717 |
|
| AFFY#1383C.zip |
MG_U74Cv2 |
GSM263718 |
|
|
|
| MyoD, wtp16, 36hr |
2482.4 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1827_1A.zip |
MG_U74Av2 |
GSM263719 |
|
| AFFY#1827_1B.zip |
MG_U74Bv2 |
GSM263720 |
|
| AFFY#1827_1C.zip |
MG_U74Cv2 |
GSM263721 |
|
|
|
| MyoD, wt841, 36hr |
2726.2 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1801A.zip |
MG_U74Av2 |
GSM263722 |
|
| AFFY#1801B.zip |
MG_U74Bv2 |
GSM263723 |
|
| AFFY#1801C.zip |
MG_U74Cv2 |
GSM263724 |
|
|
|
| Property |
Value |
| SCGP ID |
S339 |
| Name |
MyoD wt, Time Course 48hr |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 48 hr |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 48hr |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1384A.zip |
MG_U74Av2 |
GSM263725 |
|
| AFFY#1384B.zip |
MG_U74Bv2 |
GSM263726 |
|
| AFFY#1384C.zip |
MG_U74Cv2 |
GSM263727 |
|
|
|
| MyoD, wtp16, 48hr |
1724.0 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1781_1A.zip |
MG_U74Av2 |
GSM263728 |
|
| AFFY#1781_1B.zip |
MG_U74Bv2 |
GSM263729 |
|
| AFFY#1781_1C.zip |
MG_U74Cv2 |
GSM263730 |
|
|
|
| MyoD, wt841, 48hr |
1879.0 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1802A.zip |
MG_U74Av2 |
GSM263731 |
|
| AFFY#1802B.zip |
MG_U74Bv2 |
GSM263732 |
|
| AFFY#1802C.zip |
MG_U74Cv2 |
GSM263733 |
|
|
|
| Property |
Value |
| SCGP ID |
S340 |
| Name |
MyoD wt, Time Course 3 days |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 3 days |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 3 days |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1385A.zip |
MG_U74Av2 |
GSM263734 |
|
| AFFY#1385B.zip |
MG_U74Bv2 |
GSM263735 |
|
| AFFY#1385C.zip |
MG_U74Cv2 |
GSM263736 |
|
|
|
| MyoD, wtp16, 3 days |
1587.6 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1782_1A.zip |
MG_U74Av2 |
GSM263737 |
|
| AFFY#1782_1B.zip |
MG_U74Bv2 |
GSM263738 |
|
| AFFY#1782_1C.zip |
MG_U74Cv2 |
GSM263739 |
|
|
|
| MyoD, wt841, 3 days |
1467.8 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1803A.zip |
MG_U74Av2 |
GSM263740 |
|
| AFFY#1803B.zip |
MG_U74Bv2 |
GSM263741 |
|
| AFFY#1803C.zip |
MG_U74Cv2 |
GSM263742 |
|
|
|
| Property |
Value |
| SCGP ID |
S341 |
| Name |
MyoD wt, Time Course 4 days |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 4 days |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 4 days |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1386A.zip |
MG_U74Av2 |
GSM263743 |
|
| AFFY#1386B-R.zip |
MG_U74Bv2 |
GSM263744 |
|
| AFFY#1386C.zip |
MG_U74Cv2 |
GSM263745 |
|
|
|
| MyoD, wtp16, 4 days |
1166.8 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1783_1A.zip |
MG_U74Av2 |
GSM263746 |
|
| AFFY#1783_1B.zip |
MG_U74Bv2 |
GSM263747 |
|
| AFFY#1783_1C.zip |
MG_U74Cv2 |
GSM263748 |
|
|
|
| MyoD, wt841, 4 days |
2370.8 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1804A.zip |
MG_U74Av2 |
GSM263749 |
|
| AFFY#1804B-R.zip |
MG_U74Bv2 |
GSM263750 |
|
| AFFY#1804C.zip |
MG_U74Cv2 |
GSM263751 |
|
|
|
| Property |
Value |
| SCGP ID |
S342 |
| Name |
MyoD wt, Time Course 7 days |
| Description |
Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05.
The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum).
The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells.
Time point: 7 days |
| Species |
Mouse |
| Taxonomic ID |
10090 |
| Tissue of Origin |
Skeletal Muscle |
| Cell Type/Line |
Primary Myoblasts |
| Strain |
Balb/c MyoD wild Type |
| Gender |
Male |
| Growth Medium |
Ham's F10 Nutrient Mixture |
| Media Supplements |
20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM |
| Culture Conditions |
37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd. |
| RNA Prep Method |
RNeasy (Qiagen) |
| RNA Sample Type |
Total RNA |
| Cell Purification Method |
cultured |
| Contaminating Cell Type |
none |
| Estimated Purity |
100% |
Replicates
| Name |
[RNA] |
Volume |
# Cells |
Files |
Actions |
| MyoD, mb1, 7 days |
|
|
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1387A.zip |
MG_U74Av2 |
GSM263752 |
|
| AFFY#1387B.zip |
MG_U74Bv2 |
GSM263753 |
|
| AFFY#1387C.zip |
MG_U74Cv2 |
GSM263754 |
|
|
|
| MyoD, wtp16, 7 days |
1637.2 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1784A.zip |
MG_U74Av2 |
GSM263755 |
|
| AFFY#1784B.zip |
MG_U74Bv2 |
GSM263756 |
|
| AFFY#1784C.zip |
MG_U74Cv2 |
GSM263757 |
|
|
|
| MyoD, wt841, 7 days |
1142.0 ng/µL |
27µL |
More than 2 x 10E+6 Balb/c |
| Name |
Type |
GEO | Action |
| AFFY#1805_1A.zip |
MG_U74Av2 |
GSM263758 |
|
| AFFY#1805_1B.zip |
MG_U74Bv2 |
GSM263759 |
|
| AFFY#1805_1C.zip |
MG_U74Cv2 |
GSM263760 |
|
|
|
|
