Experiment View

Experiment Id: E227
Experiment Name: Differentiated Mammosphere SAGE library
Description: Differentiated mammosphere RNA contain RNA extracted from the cells of mouse mammospheres induced to differentiate over a 6-day period. Mammosphere are believed to be comprised of stem/progenitor cells.
Owner: Dr. John Hassell
Network Release Date: 2005-11-16 00:00:00
Public Release Date: 2005-11-16 00:00:00
Workflow State: RELEASED
External link: GEO Series Accession GSE3841

Sample

Property	Value
SCGP ID	S356
Name	Differentiated mammospheres
Description	Differentiated mammosphere RNA extracted from the cells of mouse mammospheres induced to differentiate over a 6-day period. Mammosphere are believed to be comprised of stem/progenitor cells.
Species	Mouse
Taxonomic ID	10090
Tissue of Origin	Mammary gland
Cell Type/Line	Differentiated mammosphere cells
Strain	FVB
Gender	Female
Karyotype	Not determined
Growth Medium	High glucose DMEM
Media Supplements	5% FBS
Culture Conditions	On collagen coated dishes 37C, 5%CO2
Defining Markers	alphaSMA-myoepithelial cells CK18-luminal epithelial cells
RNA Prep Method	Trizol
RNA Sample Type	Total RNA
Amplification Method	none
Cell Purification Method	mammospheres cultures induced to differentiate
Contaminating Cell Type	none known
Method For Estimating Purity	Use of molecular markers
Assays To Confirm Viability	Undifferentiated antecedants recapitulate mammary gland development.
Estimated Purity	50% myoepithelial, 50% luminal

Replicates

Name	[RNA]	Volume	# Cells	Files	Actions
differentiated mammospheres	2.0 ug/ul	5ul	~ 8.8x10E6	Name Type GEO Action SAGE15.zip SAGE GSM88039