Experiment View

Experiment Id: E191
Experiment Name: Sca1-CD45 sorted bone marrow
Description: Sca1-CD45 sorted bone marrow
Owner: Dr. Michael Rudnicki
Network Release Date: 2004-03-20 00:00:00
Public Release Date: 2004-11-20 00:00:00
Workflow State: RELEASED
External link: GEO Series Accession GSE3241
Download Expression Values For Experiment

Sample

Property	Value
SCGP ID	S147
Name	CPGSE#4,6,8
Description	Mouse bone marrow stained with CD45-APC & Scal-PE, sorted on Moflo for +/+ cells
Species	Mouse
Taxonomic ID	10090
Tissue of Origin	bone marrow
Cell Type/Line	haematopoeitic stem cells
Strain	C57BL/6
Gender	Female
Defining Markers	CD45+ScaI+
RNA Prep Method	Qiagen
RNA Sample Type	Total RNA
Cell Purification Method	Moflo cell sorter
Method For Estimating Purity	cell sorter reading
Analysis Requested	microarray
Estimated Purity	95-98%

Replicates

Name	[RNA]	Volume	# Cells	Files	Actions
CPGSE#4	50ng/ul	10ul	6.9 X 10^5	Name Type GEO Action Affy#275A.zip MOE430A GSM72978 Affy#275B.zip MOE430B GSM72979
CPGSE#6	50ng/ul	10ul	9.4 X 10^5	Name Type GEO Action Affy#277A.zip MOE430A GSM72980 Affy#277B.zip MOE430B GSM72981

Sample

Property	Value
SCGP ID	S233
Name	CD45-Sca1- m Bone Marrow
Description	Sorted sub-populations of mouse bone marrow. Triplicates: OGCsort#4 -/- OGCsort#5 -/- OGCsort#8 -/-
Species	Mouse
Taxonomic ID	10090
Tissue of Origin	bone marrow
Cell Type/Line	primary
Strain	C57Bl/6
Gender	Female
Karyotype	Diploid
Defining Markers	CD45- Sca1-
RNA Prep Method	RNeasy
RNA Sample Type	Total RNA
Cell Purification Method	Cell sorting
Method For Estimating Purity	Flow cytometer
Estimated Purity	90%

Replicates

Name	[RNA]	Volume	# Cells	Files	Actions
OGCsort#4 -/-				Name Type GEO Action Affy#765A.zip MOE430A GSM72982 Affy#765B.zip MOE430B GSM72983
OGCsort#5 -/-				Name Type GEO Action Affy#766A.zip MOE430A GSM72984 Affy#766B.zip MOE430B GSM72985
OGCsort#8 -/-				Name Type GEO Action Affy#767A.zip MOE430A GSM72986 Affy#767B.zip MOE430B GSM72987

Sample

Property	Value
SCGP ID	S234
Name	CD45+Sca1+ m Bone Marrow
Description	Sorted sub-populations of mouse bone marrow. Triplicates: OGCsort#1 +/+ OGCsort#5 +/+ OGCsort#6 +/+
Species	Mouse
Taxonomic ID	10090
Tissue of Origin	bone marrow
Cell Type/Line	primary
Strain	C57Bl/6
Gender	Female
Karyotype	Diploid
Defining Markers	CD45+ Sca1+
RNA Prep Method	RNeasy
RNA Sample Type	Total RNA
Cell Purification Method	Cell sorting
Method For Estimating Purity	Flow cytometer
Estimated Purity	90%

Replicates

Name	[RNA]	Volume	# Cells	Files	Actions
OGCsort#1 +/+				Name Type GEO Action Affy#768A.zip MOE430A GSM72988 Affy#768B.zip MOE430B GSM72989
OGCsort#5 +/+				Name Type GEO Action Affy#769A.zip MOE430A GSM72990 Affy#769B.zip MOE430B GSM72991
OGCsort#6 +/+				Name Type GEO Action Affy#770A.zip MOE430A GSM72992 Affy#770B.zip MOE430B GSM72993

Sample

Property	Value
SCGP ID	S235
Name	CD45+Sca1- m Bone Marrow
Description	Sorted sub-populations of mouse bone marrow. Triplicates: OGCsort#1 +/- OGCsort#7 +/- OGCsort#9 +/-
Species	Mouse
Taxonomic ID	10090
Tissue of Origin	bone marrow
Cell Type/Line	primary
Strain	C57Bl/6
Gender	Male
Karyotype	Diploid
Defining Markers	CD45+ Sca1-
RNA Prep Method	RNeasy
RNA Sample Type	Total RNA
Cell Purification Method	Cell sorting
Method For Estimating Purity	Flow cytometer
Estimated Purity	90%

Replicates

Name	[RNA]	Volume	# Cells	Files	Actions
OGCsort#1 +/-				Name Type GEO Action Affy#771A.zip MOE430A GSM72994 Affy#771B.zip MOE430B GSM72995
OGCsort#7 +/-				Name Type GEO Action Affy#772A.zip MOE430A GSM72996 Affy#772B.zip MOE430B GSM72997
OGCsort#9 +/-				Name Type GEO Action Affy#773A.zip MOE430A GSM72998 Affy#773B.zip MOE430B GSM72999