Experiment View

Experiment Id: E144
Experiment Name: Putative muscle derived stem cells from mouse.
Description: Comparison of NRA derived from SCA1+, CD45- MACS purified putative muscle derived stem cells vs. RNA derived from preplating of hind limb muscle cells.
Owner: Dr. Alain Garnier
Network Release Date: 2003-12-22 00:00:00
Public Release Date: 2004-08-22 00:00:00
Workflow State: RELEASED
External link: GEO Series Accession GSE3773
Download Expression Values For Experiment

Sample

Property	Value
SCGP ID	S184
Name	MACS #1, #2, #3, #4
Description	Putative muscle derived stem cells from mouse. Purified with MACS using Sca1 and CD45 beads. Population is Sca1+/CD45-
Species	Mouse
Taxonomic ID	10090
Tissue of Origin	Hind limb muscles
Cell Type/Line	primary culture
Strain	C57BL/10J
Gender	Female
Karyotype	BL.C5710J
Growth Medium	F10 Ham
Media Supplements	20% FBS pen/strep
Culture Conditions	37C, 5% CO2
Defining Markers	Sca1+/CD45-
RNA Prep Method	RNeasy
RNA Sample Type	Total RNA
Amplification Method	None
Cell Purification Method	MACS
Contaminating Cell Type	muscles cells
Method For Estimating Purity	FACS
Assays To Confirm Viability	erythrosin B exclusion
Estimated Purity	< 1% CD45+>80% Sca1+

Replicates

Name	[RNA]	Volume	# Cells	Files	Actions
MACS #2	55ng/ul	B - 5ul, M - 20ul	1.98x10E6	Name Type GEO Action Affy#526A.zip MOE430A GSM86581 Affy#526B.zip MOE430B GSM86582
MACS #3	65ng/ul	B - 5ul, M - 20ul	2.04x10E6	Name Type GEO Action Affy#527A.zip MOE430A GSM86583 Affy#527B.zip MOE430B GSM86584

Sample

Property	Value
SCGP ID	S197
Name	PP6 #1, #2, #3, #4
Description	Putative muscle derived stem cells purified with preplatings from muscle hind limb muscles.
Species	Mouse
Taxonomic ID	10090
Tissue of Origin	Hind limb muscles
Cell Type/Line	primary culture
Strain	C57BL/10J
Gender	Female
Karyotype	BL.C5710J
Growth Medium	F10 Ham
Media Supplements	20% FBS pen/strep
Culture Conditions	37C, 5% CO2
Defining Markers	none
RNA Prep Method	RNeasy
RNA Sample Type	Total RNA
Amplification Method	None
Cell Purification Method	preplatings
Contaminating Cell Type	muscles cells
Method For Estimating Purity	none
Assays To Confirm Viability	erythrosin B exclusion
Analysis Requested	MOE430 Affymetrix chip

Replicates

Name	[RNA]	Volume	# Cells	Files	Actions
PP6 #2	35ng/ul	B: 5ul M: 20ul	6.5x10E6	Name Type GEO Action Affy#529A.zip MOE430A GSM86585 Affy#529B.zip MOE430B GSM86586
PP6 #3	15ng/ul	B: 5ul M: 20ul	6.7x10E6	Name Type GEO Action Affy#530A.zip MOE430A GSM86587 Affy#530B.zip MOE430B GSM86588